Buffered Peptone Water is a non-selective preenrichment broth that promotes the recovery of those bacteria that may be injured. Peptone in the media supplies nitrogenous compounds needed for the growth of bacteria.
Use Buffered Peptone Water Broth as a nonselective medium for pre-enrichment of bacteria such as Listeria including L. monocytogenes, Salmonella, Cronobacter (Enterobacter), and thermophilic Campylobacter spp. The use of this nonselective broth permits recovery of bacteria after sublethal injury caused by heat, preservatives, or other Methyl Red-Voges Proskauer Buffered Glucose Broth. Use with Difco ™ /BBL ™ Voges-Proskauer Reagents A and B: Difco: 500 g/EA: 290720: Buffered Listeria Enrichment Broth Base Used for the isolation of Listeria: Difco: 500 g/EA: 212367: Buffered Peptone Water: BBL: 500 g/EA: 212345: Buffered Peptone Water: BBL: 5 lb./EA: 218104: Buffered Mar 24, 2014 · Buffered Peptone Water is used for pre-enrichment of injured salmonella species from food specimens prior to inoculation to a selective enriched medium for isolation and identification. When salmonella species are present in food, certain food processing conditions such as heat, desiccation, preservatives and pH changes may injure bacteria cells. Jan 01, 2019 · Efficacy of neutralizing buffered peptone water for recovery of Salmonella, Campylobacter, and Enterobacteriaceae from broiler carcasses at various points along a commercial immersion chilling process with peroxyacetic acid. Bourassa DV(1), Lapidus JL(1), Kennedy-Smith AE(1), Morey A(1). Use Buffered Peptone Water to prepare as a pre-enrichment medium, prior to selective enrichment in the isolation of salmonellae from foods. It also provides conditions for resuscitation of cells that have been injured by processes of food preservation.
Autoclave at 121°C for 15 min. Final pH to 7.2±0.2. Note: commercial Modified Buffered Peptone Water (3M TM dehydrated media) can be substituted for the above formulation.
Low numbers and/or sub-lethally injured Salmonella spp. cells may be present in a test food sample. Pre-enrichment in non-selective Buffered Peptone Water allows the cells time to repair and multiply before being introduced into a selective culture, improving the chances of recovering Salmonella species from the sample. Buffered Peptone Water may be used according to published standard methodologies. To comply with ISO methodology, please use Buffered Peptone Water (ISO) (CM1049) and see relevant ISO standard 8. Storage conditions and Shelf life Store dehydrated medium at 10-30°C and use before the expiry date of the label. INTENDED USE Hardy Diagnostics Peptone Water, 0.1% is recommended as a diluent for microbial enumeration purposes as described by the Food and Drug Administration and The U.S. Pharmacopeia National Formulary (USP-NF) for use in pharmaceutical and cosmetic testing and by the American Public Health Association and Environmental Protection Agency for food, dairy and water industries.
Buffered Peptone Water is a pre-enrichment medium used for sample preparation from foods prior to selective enrichment and isolation. Pre-enrichment in a non-selective medium allows for repair of cell damage and facilitates the recovery of Salmonella. Peptone 10,00 Sodium chloride 5,00 Disodium phosphate anhydrous 3,50 (*)
Dec 29, 2000 · Original Source: Bacteriological Analytical Manual, 8th Edition, Revision A, 1998. Updated and revised: 29-DEC-2000. Buffered Peptone Water is a non-selective preenrichment broth that promotes the recovery of those bacteria that may be injured. Peptone in the media supplies nitrogenous compounds needed for the growth of bacteria. Low numbers and/or sub-lethally injured Salmonella spp. cells may be present in a test food sample. Pre-enrichment in non-selective Buffered Peptone Water allows the cells time to repair and multiply before being introduced into a selective culture, improving the chances of recovering Salmonella species from the sample.